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serum free dmem  (MedChemExpress)


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    Structured Review

    MedChemExpress serum free dmem
    Serum Free Dmem, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/serum+free+dmem/DMEM/pm42319491-47-40-43
    Average 97 stars, based on 64 article reviews
    serum free dmem - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Recombinant:

    Article Title: LncRNA WFDC21P mediates the tobacco carcinogen induced malignant transformation of human normal lung epithelial cells by regulating tumor stemness.
    Article Snippet: 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a tobaccospecific carcinogen, closely linked to lung cancer development.. Cancer stem cells (CSCs) contribute to malignant tumorigenesis and progression.. Long noncoding RNAs (lncRNAs) play a crucial role in regulating cancer stemness, yet the roles and mechanisms remain unclear.

    Knockdown:

    Article Title: Intracellular protein GBF1 displays significant associations with amyloid pathology in Alzheimer's disease
    Article Snippet: .. For GBF1 knockdown, 7PA2 cells were transfected in serum‐free DMEM, using 1:1000 polyethylenimine (PEI) transfection reagent (MedChemExpress, catalog # HY‐K2014) and 1 μM GBF1 small interfering RNA (siRNA; Horizon; catalog # L‐057154‐00‐0020) SMARTPool containing the following target sequences: GCAGCAAGAGUCAUAAGUA, UCAGUGAGUUAUUGAGAAA, GGGAAGCACCCGUUAUUCA, and GAUGAGAUGUGCCGACACU. ..

    Transfection:

    Article Title: Intracellular protein GBF1 displays significant associations with amyloid pathology in Alzheimer's disease
    Article Snippet: .. For GBF1 knockdown, 7PA2 cells were transfected in serum‐free DMEM, using 1:1000 polyethylenimine (PEI) transfection reagent (MedChemExpress, catalog # HY‐K2014) and 1 μM GBF1 small interfering RNA (siRNA; Horizon; catalog # L‐057154‐00‐0020) SMARTPool containing the following target sequences: GCAGCAAGAGUCAUAAGUA, UCAGUGAGUUAUUGAGAAA, GGGAAGCACCCGUUAUUCA, and GAUGAGAUGUGCCGACACU. ..

    Small Interfering RNA:

    Article Title: Intracellular protein GBF1 displays significant associations with amyloid pathology in Alzheimer's disease
    Article Snippet: .. For GBF1 knockdown, 7PA2 cells were transfected in serum‐free DMEM, using 1:1000 polyethylenimine (PEI) transfection reagent (MedChemExpress, catalog # HY‐K2014) and 1 μM GBF1 small interfering RNA (siRNA; Horizon; catalog # L‐057154‐00‐0020) SMARTPool containing the following target sequences: GCAGCAAGAGUCAUAAGUA, UCAGUGAGUUAUUGAGAAA, GGGAAGCACCCGUUAUUCA, and GAUGAGAUGUGCCGACACU. ..

    In Vitro:

    Article Title: Phylloquinone Attenuates Oxygen-Glucose Deprivation-induced Neuronal Injury by Inhibiting Ferroptosis via the xCT/GPX4 Pathway
    Article Snippet: .. To mimic ischemia in vitro, we established the OGD (oxygen and glucose deprivation) model. HT-22 cells were washed twice with phosphate-buffered saline (PBS, pH 7.4), and then incubated in glucose-free, serum-free DMEM (C3122-0500) in a hypoxic tri-gas chamber (YF-SQ50, Yonforce, China) with resulting atmosphere consisted of 94% N2, 5% CO2 and 1% O2 at 37 °C for 2 h. After OGD induction, the culture medium was replaced: cells in the OGD group were cultured in DMEM-HG, whereas cells in the OGD + VK1 group were treated with 10 μM Phylloquinone (HY-N0684, MedChemExpress, USA) in DMEM-HG for 12 h. All experiments using HT-22 cells were independently repeated at least three times using different cell passages, and each experiment included 3 technical replicates. ..

    Article Title: Phylloquinone Attenuates Oxygen-Glucose Deprivation-induced Neuronal Injury by Inhibiting Ferroptosis via the xCT/GPX4 Pathway.
    Article Snippet: .. To mimic ischemia in vitro, we established the OGD (oxygen and glucose deprivation) model. HT-22 cells were washed twice with phosphate-buffered saline (PBS, pH 7.4), and then incubated in glucose-free, serum-free DMEM (C3122-0500) in a hypoxic tri-gas chamber (YF-SQ50, Yonforce, China) with resulting atmosphere consisted of 94% N2, 5% CO2 and 1% O2 at 37 °C for 2 h. After OGD induction, the culture medium was replaced: cells in the OGD group were cultured in DMEM-HG, whereas cells in the OGD + VK1 group were treated with 10 μM Phylloquinone (HY-N0684, MedChemExpress, USA) in DMEM-HG for 12 h. All experiments using HT-22 cells were independently repeated at least three times using different cell passages, and each experiment included 3 technical replicates. ..

    Saline:

    Article Title: Phylloquinone Attenuates Oxygen-Glucose Deprivation-induced Neuronal Injury by Inhibiting Ferroptosis via the xCT/GPX4 Pathway
    Article Snippet: .. To mimic ischemia in vitro, we established the OGD (oxygen and glucose deprivation) model. HT-22 cells were washed twice with phosphate-buffered saline (PBS, pH 7.4), and then incubated in glucose-free, serum-free DMEM (C3122-0500) in a hypoxic tri-gas chamber (YF-SQ50, Yonforce, China) with resulting atmosphere consisted of 94% N2, 5% CO2 and 1% O2 at 37 °C for 2 h. After OGD induction, the culture medium was replaced: cells in the OGD group were cultured in DMEM-HG, whereas cells in the OGD + VK1 group were treated with 10 μM Phylloquinone (HY-N0684, MedChemExpress, USA) in DMEM-HG for 12 h. All experiments using HT-22 cells were independently repeated at least three times using different cell passages, and each experiment included 3 technical replicates. ..

    Article Title: Phylloquinone Attenuates Oxygen-Glucose Deprivation-induced Neuronal Injury by Inhibiting Ferroptosis via the xCT/GPX4 Pathway.
    Article Snippet: .. To mimic ischemia in vitro, we established the OGD (oxygen and glucose deprivation) model. HT-22 cells were washed twice with phosphate-buffered saline (PBS, pH 7.4), and then incubated in glucose-free, serum-free DMEM (C3122-0500) in a hypoxic tri-gas chamber (YF-SQ50, Yonforce, China) with resulting atmosphere consisted of 94% N2, 5% CO2 and 1% O2 at 37 °C for 2 h. After OGD induction, the culture medium was replaced: cells in the OGD group were cultured in DMEM-HG, whereas cells in the OGD + VK1 group were treated with 10 μM Phylloquinone (HY-N0684, MedChemExpress, USA) in DMEM-HG for 12 h. All experiments using HT-22 cells were independently repeated at least three times using different cell passages, and each experiment included 3 technical replicates. ..

    Incubation:

    Article Title: Phylloquinone Attenuates Oxygen-Glucose Deprivation-induced Neuronal Injury by Inhibiting Ferroptosis via the xCT/GPX4 Pathway
    Article Snippet: .. To mimic ischemia in vitro, we established the OGD (oxygen and glucose deprivation) model. HT-22 cells were washed twice with phosphate-buffered saline (PBS, pH 7.4), and then incubated in glucose-free, serum-free DMEM (C3122-0500) in a hypoxic tri-gas chamber (YF-SQ50, Yonforce, China) with resulting atmosphere consisted of 94% N2, 5% CO2 and 1% O2 at 37 °C for 2 h. After OGD induction, the culture medium was replaced: cells in the OGD group were cultured in DMEM-HG, whereas cells in the OGD + VK1 group were treated with 10 μM Phylloquinone (HY-N0684, MedChemExpress, USA) in DMEM-HG for 12 h. All experiments using HT-22 cells were independently repeated at least three times using different cell passages, and each experiment included 3 technical replicates. ..

    Article Title: Astragaloside II suppresses colorectal cancer progression by upregulating LGALS4 expression.
    Article Snippet: 1 Department of Proctology, Xianyang City No.1 People’s Hospital, Xianyang 712000, Shaanxi Province, China 2 School of Public Health, Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi Province, China 3 Department of Proctology, The Second Affiliated Hospital of ShaanxiUniversity of Traditional Chinese Medicine, No. 831 Longtaiguan Road, Fengxi New City, Xixian New District, Xianyang 712000, Shaanxi Province, China Abstract This study investigates the inhibitory effect of astragaloside II (AGS-II) on colorectal cancer (CRC) progression and elucidates the underlying mechanism involving LGALS4.. The effects of AGS-II on proliferation, migration, and apoptosis of CRC cells were assessed using cell counting kit-8, colony formation, scratch wound, and flow cytometry assays.. Potential targets were screened through integrated bioinformatics analysis and validated via RT-qPCR and Western blot.

    Article Title: Smart Gated Hollow Mesoporous Silica Hydrogel for Targeting Endoplasmic Reticulum Stress and Promoting Periodontal Tissue Regeneration
    Article Snippet: .. After a 24‐h attachment period, the cells were exposed to HP@TF127, HQUP@F127, HQUP@TF127 in serum‐free DMEM for 48 h. The assessment of metabolic activity utilized CCK‐8 reagent (MedChemExpress); specifically, 10% v/v CCK‐8 solution was introduced to each well, incubated for 1 h in the dark at 37 °C, and absorbance readings were taken at 450 nm using a SpectraMax CMax Plus microplate reader (Molecular Devices). ..

    Article Title: Phylloquinone Attenuates Oxygen-Glucose Deprivation-induced Neuronal Injury by Inhibiting Ferroptosis via the xCT/GPX4 Pathway.
    Article Snippet: .. To mimic ischemia in vitro, we established the OGD (oxygen and glucose deprivation) model. HT-22 cells were washed twice with phosphate-buffered saline (PBS, pH 7.4), and then incubated in glucose-free, serum-free DMEM (C3122-0500) in a hypoxic tri-gas chamber (YF-SQ50, Yonforce, China) with resulting atmosphere consisted of 94% N2, 5% CO2 and 1% O2 at 37 °C for 2 h. After OGD induction, the culture medium was replaced: cells in the OGD group were cultured in DMEM-HG, whereas cells in the OGD + VK1 group were treated with 10 μM Phylloquinone (HY-N0684, MedChemExpress, USA) in DMEM-HG for 12 h. All experiments using HT-22 cells were independently repeated at least three times using different cell passages, and each experiment included 3 technical replicates. ..

    Cell Culture:

    Article Title: Phylloquinone Attenuates Oxygen-Glucose Deprivation-induced Neuronal Injury by Inhibiting Ferroptosis via the xCT/GPX4 Pathway
    Article Snippet: .. To mimic ischemia in vitro, we established the OGD (oxygen and glucose deprivation) model. HT-22 cells were washed twice with phosphate-buffered saline (PBS, pH 7.4), and then incubated in glucose-free, serum-free DMEM (C3122-0500) in a hypoxic tri-gas chamber (YF-SQ50, Yonforce, China) with resulting atmosphere consisted of 94% N2, 5% CO2 and 1% O2 at 37 °C for 2 h. After OGD induction, the culture medium was replaced: cells in the OGD group were cultured in DMEM-HG, whereas cells in the OGD + VK1 group were treated with 10 μM Phylloquinone (HY-N0684, MedChemExpress, USA) in DMEM-HG for 12 h. All experiments using HT-22 cells were independently repeated at least three times using different cell passages, and each experiment included 3 technical replicates. ..

    Article Title: Phylloquinone Attenuates Oxygen-Glucose Deprivation-induced Neuronal Injury by Inhibiting Ferroptosis via the xCT/GPX4 Pathway.
    Article Snippet: .. To mimic ischemia in vitro, we established the OGD (oxygen and glucose deprivation) model. HT-22 cells were washed twice with phosphate-buffered saline (PBS, pH 7.4), and then incubated in glucose-free, serum-free DMEM (C3122-0500) in a hypoxic tri-gas chamber (YF-SQ50, Yonforce, China) with resulting atmosphere consisted of 94% N2, 5% CO2 and 1% O2 at 37 °C for 2 h. After OGD induction, the culture medium was replaced: cells in the OGD group were cultured in DMEM-HG, whereas cells in the OGD + VK1 group were treated with 10 μM Phylloquinone (HY-N0684, MedChemExpress, USA) in DMEM-HG for 12 h. All experiments using HT-22 cells were independently repeated at least three times using different cell passages, and each experiment included 3 technical replicates. ..

    CCK-8 Assay:

    Article Title: Astragaloside II suppresses colorectal cancer progression by upregulating LGALS4 expression.
    Article Snippet: 1 Department of Proctology, Xianyang City No.1 People’s Hospital, Xianyang 712000, Shaanxi Province, China 2 School of Public Health, Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi Province, China 3 Department of Proctology, The Second Affiliated Hospital of ShaanxiUniversity of Traditional Chinese Medicine, No. 831 Longtaiguan Road, Fengxi New City, Xixian New District, Xianyang 712000, Shaanxi Province, China Abstract This study investigates the inhibitory effect of astragaloside II (AGS-II) on colorectal cancer (CRC) progression and elucidates the underlying mechanism involving LGALS4.. The effects of AGS-II on proliferation, migration, and apoptosis of CRC cells were assessed using cell counting kit-8, colony formation, scratch wound, and flow cytometry assays.. Potential targets were screened through integrated bioinformatics analysis and validated via RT-qPCR and Western blot.

    Activity Assay:

    Article Title: Smart Gated Hollow Mesoporous Silica Hydrogel for Targeting Endoplasmic Reticulum Stress and Promoting Periodontal Tissue Regeneration
    Article Snippet: .. After a 24‐h attachment period, the cells were exposed to HP@TF127, HQUP@F127, HQUP@TF127 in serum‐free DMEM for 48 h. The assessment of metabolic activity utilized CCK‐8 reagent (MedChemExpress); specifically, 10% v/v CCK‐8 solution was introduced to each well, incubated for 1 h in the dark at 37 °C, and absorbance readings were taken at 450 nm using a SpectraMax CMax Plus microplate reader (Molecular Devices). ..



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